Rab3D is critical for secretory granule maturation in PC12 cells.

Rab3D is critical for secretory granule maturation in PC12 cells.

Blog Article

Neuropeptide- and hormone-containing secretory granules (SGs) are synthesized at the trans-Golgi network (TGN) as immature secretory granules (ISGs) and complete their maturation Toner Originals in the F-actin-rich cell cortex.This maturation process is characterized by acidification-dependent processing of cargo proteins, condensation of the SG matrix and removal of membrane and proteins not destined to mature secretory granules (MSGs).Here we addressed a potential role of Rab3 isoforms in these maturation steps by expressing their nucleotide-binding deficient mutants in PC12 cells.Our data show that the presence of Rab3D(N135I) decreases the restriction of maturing SGs to the F-actin-rich cell cortex, blocks the removal of the endoprotease furin from SGs and impedes the processing of the luminal Bike Parts - BMX - Wheel Rims SG protein secretogranin II.This strongly suggests that Rab3D is implicated in the subcellular localization and maturation of ISGs.